Rescue in vitro maturation using ovarian support cells of human oocytes from conventional stimulation cycles yields oocytes with improved nuclear maturation and transcriptomic resemblance to in vivo matured oocytes
Purpose: Determine if the gene expression profles of ovarian support cells (OSCs) and cumulus-free oocytes are bidirectionally infuenced by co-culture during in vitro maturation (IVM).
Methods: Fertility patients aged 25 to 45 years old undergoing conventional ovarian stimulation donated denuded immature oocytes for research. Oocytes were randomly allocated to either OSC-IVM culture (intervention) or Media-IVM culture (control) for 24–28 h. The OSC-IVM culture condition was composed of 100,000 OSCs in suspension culture with human chorionic gonadotropin (hCG), recombinant follicle stimulating hormone (rFSH), androstenedione, and doxycycline supplementation. The Media-IVM control lacked OSCs and contained the same supplementation. A limited set of in vivo matured
MII: oocytes were donated for comparative evaluation. Endpoints consisted of MII formation rate, morphological and spindle quality assessment, and gene expression analysis compared to in vitro and in vivo controls.
Results: OSC-IVM resulted in a statistically signifcant improvement in MII formation rate compared to the Media-IVM
control, with no apparent efect on morphology or spindle assembly. OSC-IVM MII oocytes displayed a closer transcriptomic maturity signature to IVF-MII controls than Media-IVM control MII oocytes. The gene expression profle of OSCs was modulated in the presence of oocytes, displaying culture- and time-dependent diferential gene expression during IVM.
Conclusion: The OSC-IVM platform is a novel tool for rescue maturation of human oocytes, yielding oocytes with improved nuclear maturation and a closer transcriptomic resemblance to in vivo matured oocytes, indicating a potential enhancement in oocyte cytoplasmic maturation. These improvements on oocyte quality after OSC-IVM are possibly occurring through bidirectional crosstalk of cumulus-free oocytes and ovarian support cells.
Bruna Paulsen, Sabrina Piechota, Ferran Barrachina, Alexa Giovannini, Simone Kats, Kathryn S. Potts, Graham Rockwell, Maria Marchante, Samantha L. Estevez, Alexander D. Noblett, Alexandra B. Figueroa, Caroline Aschenberger, Dawn A. Kelk, Marcy Forti, Shelby Marcinyshyn, Klaus Wiemer, Marta Sanchez, Pedro Belchin, Joseph A. Lee, Erkan Buyuk, Rick E. Slifkin, Merrick Pierson Smela, Patrick R. J. Fortuna, Pranam Chatterjee, David H. McCulloh, Alan B. Copperman, Daniel Ordonez‐Perez, Joshua U. Klein, Christian C. Kramme
Journal of Assisted Reproduction and Genetics. doi: 10.1007/s10815-024-03143-4.